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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which leads to chromosome instability, and thus may contribute to tumorigenesis. This protein was observed to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a essential position in advertising and marketing mobile-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually described. Two supplemental splice variants had been described but in-depth nucleotide sequence info will not be yet out there.
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Blocking peptides are peptides that bind specifically on the goal antibody and block antibody binding. These peptide ordinarily has the epitope acknowledged because of the antibody.
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This tutorial outlines even more achievable optimization of mobile and immunoassay parameters to guarantee the absolute best final results are...
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This guideline outlines even further feasible optimization of mobile and immunoassay parameters to be certain the best possible outcomes are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining with the blocked antibody versus the antibody by itself, you can see which staining is certain.
Goal Information Cyclin E belongs into the remarkably conserved cyclin loved ones, whose members are characterized by a remarkable periodicity in protein abundance through the cell cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation designs which add to your temporal coordination of each mitotic party. Cyclin E kinds a complex with and features to be a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary which is degraded as cells progress as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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